Proteins interact with each other and organize themselves into macromolecular machines (ie. complexes) to carry out essential functions of the cell. We have a good understanding of a few complexes such as the proteasome and the ribosome but currently we have an incomplete view of all protein complexes as well as their functions. The hu.MAP attempts to address this lack of understanding by integrating several large scale protein interaction datasets to obtain the most comprehensive view of protein complexes. Specifically, we integrated two large scale affinity purification mass spectrometry (AP/MS) datasets from Bioplex and Hein et al. with our dataset of large scale biochemical fractionations (Wan et al) and produced a complex map with over 4k complexes.

Contributers

  Kevin Drew (website)
  Edward Marcotte (website)

Funding

  NIH F32 GM112495

Citation

• Kevin Drew, Chanjae Lee, Ryan L Huizar, Fan Tu, Blake Borgeson, Claire D McWhite, Yun Ma, John B Wallingford, Edward M Marcotte Integration of over 9,000 mass spectrometry experiments builds a global map of human protein complexes. Molecular Systems Biology (2017) 13, 932. DOI 10.15252/msb.20167490

References

• Wan, Borgeson et al. Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44. doi: 10.1038/nature14877. Epub 2015 Sep 7.
• Hein et al. A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23. doi: 10.1016/j.cell.2015.09.053. Epub 2015 Oct 22.
• Huttlin et al. The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell. 2015 Jul 16;162(2):425-40. doi: 10.1016/j.cell.2015.06.043.
• Reimand et al. g:Profiler-a web server for functional interpretation of gene lists (2016 update). Nucleic Acids Res. 2016 Jul 8;44(W1):W83-9. doi: 10.1093/nar/gkw199.

Contact

  proteincomplexmap (at) gmail.com